PSMA | HUABIO HA721593 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PSMA

catalog :

HA721593

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE42-45

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PSMA on different lysates with Rabbit anti-PSMA antibody (HA721593) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721593) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-PSMA antibody (HA721593) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721593) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721593

Target name :

PSMA

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PSMA aa 1-150 / 750.

Uniprot id :

Q04609>SwissProt: Q04609 Human

Host :

Rabbit

Clone number :

JE42-45

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

LNCaP cell lysate, 22RV1 cell lysate, human prostate carcinoma tissue.

Molecular wt :

Predicted band size: 84 kDa

Subcellular location :

Cell membrane; Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage