product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-Beta Catenin (S33 + S37)
catalog :
HA721580
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE59-59
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Phospho-Beta Catenin (S33 + S37) on different lysates with Rabbit anti-Phospho-Beta Catenin (S33 + S37) antibody (HA721580) at 1/1,000 dilution.
Lane 1: 293T whole cell lysate
Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate
Lane 3: C6 whole cell lysate
Lane 4: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721580
Target name :
Phospho-Beta Catenin (S33 + S37)
Species reactivity :
Human,Rat,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic phosphopeptide corresponding to residues surrounding Ser33 and Ser37 of human β-catenin.
Uniprot id :
P35222>SwissProt: P35222 Human;SwissProt: Q02248 Mouse;SwissProt: Q9WU82 Rat
Host :
Rabbit
Clone number :
JE59-59
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T treated with 100nM Calyculin A for 15 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate.
Molecular wt :
Predicted band size: 85 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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