CD30 | HUABIO HA721579 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD30

catalog :

HA721579

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE57-71

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD30 on different lysates with Rabbit anti-CD30 antibody (HA721579) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: Raji cell lysate (negative) Lane 3: Daudi cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 64 kDa Observed band size: 120 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721579) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Rabbit anti-CD30 antibody (HA721579) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721579) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721579

Target name :

CD30

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD30 aa 546-595 / 595.

Uniprot id :

P28908>SwissProt: P28908 Human

Host :

Rabbit

Clone number :

JE57-71

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HDLM-2 cell lysate, human hodgkin lymphoma tissue.

Molecular wt :

Predicted band size: 64 kDa

Subcellular location :

Cell membrane; Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage