Fyn | HUABIO HA721577 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Fyn

catalog :

HA721577

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE42-22

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution. Lane 1: Rat testis tissue lysate Lane 2: Mouse testis tissue lysate Lane 3: Human brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 61 kDa Observed band size: 59 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721577

Target name :

Fyn

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Fyn aa 25-74 / 537.

Uniprot id :

P06241>SwissProt: P06241 Human;SwissProt: P39688 Mouse;SwissProt: Q62844 Rat

Host :

Rabbit

Clone number :

JE42-22

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Rat testis tissue lysate, Mouse testis tissue lysate, Human brain tissue lysate, Mouse lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, 293T cell lysate, NIH/3T3 cell lysate.

Molecular wt :

Predicted band size: 61 kDa

Subcellular location :

Cytoplasm, Nucleus, Cell membrane, Perikaryon.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721577_4.jpg

Pic legend4 :

Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (HA721577) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Fyn KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 59 kDa Exposure time: 180 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage