GFPT1 | HUABIO HA721554 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GFPT1

catalog :

HA721554

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE43-68

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GFPT1 on different lysates with Rabbit anti-GFPT1 antibody (HA721554) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: Huh7 cell lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (40 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721554) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-GFPT1 antibody (HA721554) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721554) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GFPT1 antibody (HA721554) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721554) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721554

Target name :

GFPT1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human GFPT1 aa 600-699.

Uniprot id :

Q06210>SwissProt: Q06210 Human;SwissProt: P47856 Mouse;SwissProt: P82808 Rat

Host :

Rabbit

Clone number :

JE43-68

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HCT 116 cell lysate, Huh7 cell lysate, Mouse testis tissue lysate, Rat spleen tissue lysate, human colon carcinoma tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 79 kDa

Subcellular location :

Cytosol

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200-1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721554_4.jpg

Pic legend4 :

Western blot analysis of GFPT1 on different lysates with Rabbit anti-GFPT1 antibody (HA721554) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si GFPT1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 1 minute 7 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721554) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage