antibody

NF-L

HA721538

100μl

385.00 USD

monoclonal

domestic rabbit

nonconjugated

PS02-10

human, mouse, rat

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

NF-L

Human,Mouse,Rat,Cynomolgus monkey,Pig

WB,IHC-P,IF-Cell,IF-Tissue,IHC-Fr

Non-conjugated

Recombinant protein.

P07196>SwissProt: P07196 Human;SwissProt: P08551 Mouse;SwissProt: P19527 Rat

Rabbit

PS02-10

IgG

100μl

385.00 USD

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

Mouse brain tissue lysate, rat brain tissue lysate, mouse hippocampus tissue lysate, rat hippocampus tissue lysate, human brain tissue, mouse brain tissue, rat cerebellum tissue, SH-SY5Y, mouse cerebral cortex tissue, mouse hippocampus tissue, mouse glia cells.

Predicted band size: 62 kDa

Cell projection, axon, Cytoplasm, cytoskeleton.

1 mg/mL.

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:500 ;IHC-Fr: 1:500

Relative expression (RE)

https://storage.huabio.cn/huabio/productImg/HA721538_4.jpg

Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1:500

https://storage.huabio.cn/huabio/productImg/HA721538_5.jpg

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (HA721538) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721538) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA721538_6.jpg

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NF-L antibody (HA721538) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721538) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA721538_7.jpg

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-NF-L antibody (HA721538) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721538) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA721538_8.jpg

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NF-L antibody (HA721538) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721538) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA721538_9.jpg

Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (HA721538) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (HA721538) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

https://storage.huabio.cn/huabio/productImg/HA721538_10.jpg

Immunocytochemistry analysis of mouse glial cells labeling NF-L with Rabbit anti-NF-L antibody (HA721538) at 1/1,000 dilution. Cells were fixed with 4% PFA (15 min), permeabilized with 0.25% TritonX-100 for 15 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4℃ with Rabbit anti-NF-L antibody (HA721538) at at 1/1,000 dilution. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.