SEC62 | HUABIO HA721534 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SEC62

catalog :

HA721534

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE62-80

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (HA721534) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: A431 cell lysate Lane 6: HEK-293 cell lysate Lane 7: PANC-1 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721534) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SEC62 antibody (HA721534) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721534) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SEC62 antibody (HA721534) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721534) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721534

Target name :

SEC62

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human SEC62 aa256-350.

Uniprot id :

Q99442>SwissProt: Q99442 Human;SwissProt: Q8BU14 Mouse;Entrez Gene: 294912 Rat

Host :

Rabbit

Clone number :

JE62-80

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, K-562 cell lysate, A431 cell lysate, HEK-293 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, HeLa.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Endoplasmic reticulum membrane.

Concentration :

1 mg/ml

Recommended dilutions :

WB: 1:1,000 ;IHC: 1:1,000-1:3,000 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721534_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SEC62 antibody (HA721534) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721534) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721534_5.jpg

Pic legend5 :

Flow cytometric analysis of HeLa cells labeling SEC62. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721534, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage