F4/80 | HUABIO HA721520 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

F4/80

catalog :

HA721520

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-85

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate (no heat) (40 µg/Lane) Lane 2: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 4: Rat kidney tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane) Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 (F4/80, HA721520, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green) Antigen retrieval: Not required

product information

SKU :

HA721520

Target name :

F4/80

Species reactivity :

Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IHC-Fr,mIHC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse F4/80 aa 1-650 / 931.

Uniprot id :

Q61549>SwissProt: Q61549 Mouse;SwissProt: Q5Y4N8 Rat

Host :

Rabbit

Clone number :

PSH0-85

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse kidney tissue lysate, RAW264.7 cell lysate, Rat kidney tissue lysate, Rat spleen tissue lysate, mouse liver tissue, mouse spleen tissue, rat liver tissue, rat spleen tissue, mouse lung tissue, rat lung tissue.

Molecular wt :

Predicted band size: 102 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IF-Tissue: 1:500 ;IHC-Fr: 1:1,000 ;mIHC: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721520_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Rat Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 (F4/80, HA721520, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green) Antigen retrieval: Not required

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721520_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721520_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA721520_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA721520_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA721520_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA721520_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA721520_11.jpg

Pic legend11 :

Application: IF-tissue Species: Mouse Site: Liver Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA721520_12.jpg

Pic legend12 :

Application: IF-tissue Species: Rat Site: Liver Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA721520_13.jpg

Pic legend13 :

Application: IF-tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img14 :

https://storage.huabio.cn/huabio/productImg/HA721520_14.jpg

Pic legend14 :

Application: IF-tissue Species: Mouse Site: small intestine Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img15 :

https://storage.huabio.cn/huabio/productImg/HA721520_15.jpg

Pic legend15 :

mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (HA721520) at 1/500 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

more info or order :

HUABIO product webpage