CEACAM1 | HUABIO HA721507 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CEACAM1

catalog :

HA721507

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-78

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution and Mouse anti-6X His-tag antibody (HA600030) at 1/1,000 dilution. Lane 1: His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng Lane 2: His-tagged human CEACAM-5 recombinant protein aa (aa35-422) 20ng Lane 3: His-tagged human CEACAM-6 recombinant protein aa (aa35-326) 20ng Lane 4: His-tagged human CEACAM-8 recombinant protein aa (aa35-335) 20ng Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721507) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: SW480 cell lysate (no heat) (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (no heat) (negative) (20 µg/Lane) Lane 5: 293T cell lysate (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 58 kDa Observed band size: 58-150 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721507) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721507) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721507

Target name :

CEACAM1

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CEACAM1 aa 35-428 / 526.

Uniprot id :

P13688>SwissProt: P13688 Human

Host :

Rabbit

Clone number :

PSH0-78

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SW480 cell lysate, A549 cell lysate, human kidney tissue, human liver tissue.

Molecular wt :

Predicted band size: 58 kDa

Subcellular location :

Cell membrane, Lateral cell membrane, Apical cell membrane, Basal cell membrane, Cell junction, adherens junction.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721507_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721507) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage