SAP97 | HUABIO HA721502 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SAP97

catalog :

HA721502

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE39-74

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SAP97 on different lysates with Rabbit anti-SAP97 antibody (HA721502) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of SAP97 on different lysates with Rabbit anti-SAP97 antibody (HA721502) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD SAP97 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721502) at 1/2,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SAP97 antibody (HA721502) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721502) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721502

Target name :

SAP97

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N terminal Human SAP97.

Uniprot id :

Q12959>SwissProt: Q12959 Human;SwissProt: Q811D0 Mouse;SwissProt: Q62696 Rat

Host :

Rabbit

Clone number :

JE39-74

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, HepG2 cell lysate, U-87 MG cell lysate, SiHa cell lysate, NCI-H226 cell lysate, NIH/3T3 cell lysate, mouse liver tissue, rat cerebellum tissue, SiHa.

Molecular wt :

Predicted band size: 100 kDa

Subcellular location :

Membrane, Basolateral cell membrane, Endoplasmic reticulum membrane, Postsynaptic density, Synapse, Cell membrane, sarcolemma, Apical cell membrane, Cell junction, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200 ;FC: 1:500-1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721502_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SAP97 antibody (HA721502) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721502) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721502_5.jpg

Pic legend5 :

Flow cytometric analysis of SiHa cells labeling SAP97. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721502, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage