Hsp70 | HUABIO HA721497 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Hsp70

catalog :

HA721497

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PD01-65

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Hsp70 on different lysates with Rabbit anti-Hsp70 antibody (HA721497) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721497) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IF-Tissue Species: Human Site: breast carcinoma Sample: Paraffin-embedded section Antibody concentration: 1/200

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Hsp70 antibody (HA721497) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721497) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721497

Target name :

Hsp70

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant fragment within Human Hsp70 aa 50-300.

Uniprot id :

P0DMV9>SwissProt: P0DMV9 Human;SwissProt: P0DMV8 Human;SwissProt: P17879 Mouse;SwissProt: Q61696 Mouse;SwissProt: Q07439 Rat

Host :

Rabbit

Clone number :

PD01-65

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HCT 116 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, A431 cell lysate, A549 cell lysate, human breast carcinoma tissue, human liver carcinoma tissue.

Molecular wt :

Predicted band size: 70 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721497_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Hsp70 antibody (HA721497) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721497) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage