Pumilio 2 | HUABIO HA721486 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Pumilio 2

catalog :

HA721486

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE37-76

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Pumilio 2 on different lysates with Rabbit anti-Pumilio 2 antibody (HA721486) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 114 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721486) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of U-2 OS cells labeling Pumilio 2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721486, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA721486

Target name :

Pumilio 2

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Pumilio 2 aa 1-100.

Uniprot id :

Q8TB72>SwissProt: Q8TB72 Human

Host :

Rabbit

Clone number :

JE37-76

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, U-2 OS.

Molecular wt :

Predicted band size: 114 kDa

Subcellular location :

Cytoplasm, Cytoplasmic granule, perinuclear region.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1:500-1:1,000

more info or order :

HUABIO product webpage