BCAR1 | HUABIO HA721465 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BCAR1

catalog :

HA721465

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE36-56

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BCAR1 on different lysates with Rabbit anti-BCAR1 antibody (HA721465) at 1/1,000 dilution. Lane 1: HUVEC cell lysate Lane 2: HeLa cell lysate Lane 3: U-2 OS cell lysate Lane 4: HEK-293 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721465) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of BCAR1 on different lysates with Rabbit anti-BCAR1 antibody (HA721465) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-BCAR1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 93 kDa Observed band size: 110 kDa Exposure time: 94 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721465) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721465

Target name :

BCAR1

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human BCAR1 aa 1-100 / 870.

Uniprot id :

P56945>SwissProt: P56945 Human;SwissProt: Q61140 Mouse;SwissProt: Q63767 Rat

Host :

Rabbit

Clone number :

JE36-56

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HUVEC cell lysate, HeLa cell lysate, U-2 OS cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.

Molecular wt :

Predicted band size: 93 kDa

Subcellular location :

Cell junction, focal adhesion, Cytoplasm, Cell projection, axon.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage