product summary
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company name :
HUABIO
product type :
antibody
product name :
Cytokeratin 5+6
catalog :
HA721455
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH0-67
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 2 :
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA721455
Target name :
Cytokeratin 5+6
Species reactivity :
Human,Mouse,Rat
Applications :
IHC-P,IF-Tissue,WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human Cytokeratin 6 186-235.
Uniprot id :
P02538>SwissProt: P02538 Human;SwissProt: P04259 Human;SwissProt: P13647 Human
Host :
Rabbit
Clone number :
PSH0-67
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Human breast tissue, mouse skin tissue, rat skin tissue, mouse brain tissue lysate, rat brain tissue lysate, human lung squamous cell carcinoma tissue, human skin tissue, human tonsil tissue.
Molecular wt :
Predicted band size: 60 kDa
Subcellular location :
Cytoskeleton
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-P: 1:200-1:1,000
;IF-Tissue: 1:1,000
;WB: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721455_4.jpg
Pic legend4 :
Western blot analysis of Cytokeratin 5+6 on different lysates with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.
Lane 1: Mouse skin tissue lysate (hot lysis)
Lane 2: Rat skin tissue lysate (hot lysis)
Lane 3: Rat skin tissue lysate (RIPA lysis)
Lysates/proteins at 40 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721455) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA721455_5.jpg
Pic legend5 :
Immunofluorescence analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling Cytokeratin 5+6 with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721455, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA721455_6.jpg
Pic legend6 :
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5+6 with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721455, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA721455_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA721455_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA721455_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/HA721455_10.jpg
Pic legend10 :
Immunohistochemical analysis of paraffin embedded human lung squamous cell carcinoma tissue using anti-Cytokeratin 5+6 antibody (1/1,000) performed on the Leica® BOND™ RX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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