FRA1 | HUABIO HA721447 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FRA1

catalog :

HA721447

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE33-20

reactivity :

human

application :

western blot, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FRA1 on different lysates with Rabbit anti-FRA1 antibody (HA721447) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (negative) (20 µg/Lane) Lane 5: HEK-293 cell lysate (negative) (20 µg/Lane) Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721447) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-FRA1 antibody (HA721447) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721447) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-FRA1 antibody (HA721447) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721447) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721447

Target name :

FRA1

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Cell,FC,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human FRA1 aa 121-220 / 271.

Uniprot id :

P15407>SwissProt: P15407 Human

Host :

Rabbit

Clone number :

JE33-20

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MDA-MB-231 cell lysate, U-87 MG cell lysate, A431 cell lysate, human cervical carcinoma tissue, human small intestine tissue, U-87 MG.

Molecular wt :

Predicted band size: 29 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,500-1:3,000 ;IF-Cell: 1:100 ;FC: 1:500-1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721447_4.jpg

Pic legend4 :

Immunocytochemistry analysis of U-87 MG cells labeling FRA1 with Rabbit anti-FRA1 antibody (HA721447) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FRA1 antibody (HA721447) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721447_5.jpg

Pic legend5 :

Flow cytometric analysis of U-87 MG cells labeling FRA1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721447, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721447_6.jpg

Pic legend6 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from MDA-MB-231 cells with FRA1 (HA721447) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage