Phospho-ULK1 (S556) | HUABIO HA721445 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-ULK1 (S556)

catalog :

HA721445

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE59-28

reactivity :

human, mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-ULK1 (S556) on different lysates with Rabbit anti-Phospho-ULK1 (S556) antibody (HA721445) at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate Lane 2: MCF7 treated with 0.5μM Oligomycin for 30 minutes whole cell lysate Lane 3: MCF7 treated with λpp for 1 hour whole cell lysate Lane 4: MCF7 treated with 0.5μM Oligomycin for 30 minutes, then treated with λpp for 1 hour whole cell lysate Lysates/proteins at 14 µg/Lane. Predicted band size: 113 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721445) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721445

Target name :

Phospho-ULK1 (S556)

Species reactivity :

Human,Mouse

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser555 of mouse ULK1 protein.

Uniprot id :

O75385>SwissProt: O75385 Human;SwissProt: O70405 Mouse

Host :

Rabbit

Clone number :

JE59-28

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 whole cell lysate, MCF7 treated with 0.5μM Oligomycin for 30 minutes whole cell lysate.

Molecular wt :

Predicted band size: 113 kDa

Subcellular location :

Cytoplasm, cytosol, Preautophagosomal structure.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage