product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-HER2 / ErbB2 (Y1221 + Y1222)
catalog :
HA721433
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE44-12
reactivity :
human
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA721433 image 1
Western blot analysis of Phospho-HER2 / ErbB2 (Y1221 + Y1222) on different lysates with Rabbit anti-Phospho-HER2 / ErbB2 (Y1221 + Y1222) antibody (HA721433) at 1/1,000 dilution. Lane 1: SK-Br-3 whole cell lysate Lane 2: SK-Br-3 treated with λpp for 1 hour whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 138 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721433) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721433
Target name :
Phospho-HER2 / ErbB2 (Y1221 + Y1222)
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic phosphopeptide corresponding to residues surrounding tyrosines 1221/1222 of human ErbB2 protein
Uniprot id :
P04626>SwissProt: P04626 Human
Host :
Rabbit
Clone number :
JE44-12
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SK-Br-3 whole cell lysate.
Molecular wt :
Predicted band size: 138 kDa
Subcellular location :
Cell membrane, Nucleus, Cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Cell treatment (CT)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!