product summary
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company name :
HUABIO
product type :
antibody
product name :
PAR2
catalog :
HA721426
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE35-05
reactivity :
human, mouse
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody (HA721426) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: PANC-1 cell lysate (15 µg/Lane)
Lane 4: PC-3M cell lysate (15 µg/Lane)
Lane 5: AGS cell lysate (15 µg/Lane)
Lane 6: SGC-7901 cell lysate (15 µg/Lane)
Lane 7: Human kidney tissue lysate (30 µg/Lane)
Lane 8: Human stomach tissue lysate (30 µg/Lane)
Lane 9: Mouse large intestine tissue lysate (30 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 55 kDa (Glycosylation)
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721426) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
Flow cytometric analysis of HepG2 cells labeling PAR2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721426, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA721426
Target name :
PAR2
Species reactivity :
Human,Mouse
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human PAR2 aa 328-377 / 397.
Uniprot id :
P55085>SwissProt: P55085 Human;SwissProt: P55086 Mouse
Host :
Rabbit
Clone number :
JE35-05
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HCT 116 cell lysate, HepG2 cell lysate, PANC-1 cell lysate, PC-3M cell lysate, AGS cell lysate, SGC-7901 cell lysate, human kidney tissue lysate, human stomach tissue lysate, mouse large intestine tissue lysate, HepG2.
Molecular wt :
Predicted band size: 44 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;FC: 1:500-1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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