product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
Desmocollin 1
catalog :
HA721418
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE66-57
reactivity :
mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Desmocollin 1 on different lysates with Rabbit anti-Desmocollin 1 antibody (HA721418) at 1/1,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: L6 cell lysate
Lane 3: RH-35 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721418) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Desmocollin 1 on different lysates with Rabbit anti-Desmocollin 1 antibody (HA721418) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Mouse heart tissue lysate
Lane 4: Rat heart tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721418) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721418
Target name :
Desmocollin 1
Species reactivity :
Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Desmocollin 1 aa 795-894 / 894.
Uniprot id :
P55849>SwissProt: P55849 Mouse;Entrez Gene: 291759 Rat
Host :
Rabbit
Clone number :
JE66-57
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
C2C12 cell lysate, L6 cell lysate, RH-35 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, mouse heart tissue lysate, rat heart tissue lysate.
Molecular wt :
Predicted band size: 100 kDa
Subcellular location :
Cell membrane, Cell junction, desmosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments