Slit2 | HUABIO HA721407 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Slit2

catalog :

HA721407

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE31-87

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (HA721407) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 µg/Lane) Lane 2: 293T cell lysate (15 µg/Lane) Lane 3: PC-3M cell lysate (15 µg/Lane) Lane 4: Raji cell lysate (15 µg/Lane) Lane 5: HCT 116 cell lysate (15 µg/Lane) Lane 6: HeLa cell lysate (15 µg/Lane) Lane 7: Rat pancreas tissue lysate (30 µg/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721407) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (HA721407) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: Human brain tissue lysate (30 µg/Lane) Lane 3: Mouse brain tissue lysate (30 µg/Lane) Lane 4: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721407) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (HA721407) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (HA721407) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA721407

Target name :

Slit2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Slit2 aa 1,380-1,529 / 1,529.

Uniprot id :

O94813>SwissProt: O94813 Human;SwissProt: Q9R1B9 Mouse;SwissProt: Q9WVC1 Rat

Host :

Rabbit

Clone number :

JE31-87

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, 293T cell lysate, PC-3M cell lysate, Raji cell lysate, HCT 116 cell lysate, HeLa cell lysate, rat pancreas tissue lysate, SH-SY5Y cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, A549.

Molecular wt :

Predicted band size: 170 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:50-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721407_4.jpg

Pic legend4 :

Flow cytometric analysis of A549 cells labeling Slit2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721407, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage