product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
Chromogranin B
catalog :
HA721398
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE31-72
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (HA721398) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: SW620 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 120 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721398) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (HA721398) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: RAW264.7 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 120 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721398) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721398
Target name :
Chromogranin B
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Chromogranin B aa 601-650 / 677.
Uniprot id :
P05060>SwissProt: P05060 Human;SwissProt: P16014 Mouse;SwissProt: O35314 Rat
Host :
Rabbit
Clone number :
JE31-72
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T cell lysate, SH-SY5Y cell lysate, SW620 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, HeLa cell lysate.
Molecular wt :
Predicted band size: 78 kDa
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments