RAB22A | HUABIO HA721387 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RAB22A

catalog :

HA721387

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE66-08

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-RAB22A KD cell lysate (10 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: K-562 cell lysate (15 µg/Lane) Lane 3: HeLa cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: Jurkat cell lysate (15 µg/Lane) Lane 6: U-2 OS cell lysate (15 µg/Lane) Lane 7: A549 cell lysate (15 µg/Lane) Lane 8: HEK-293 cell lysate (15 µg/Lane) Lane 9: Mouse brain tissue lysate (30 µg/Lane) Lane 10: Rat brain tissue lysate (30 µg/Lane) Lane 11: Mouse kidney tissue lysate (30 µg/Lane) Lane 12: Mouse testis tissue lysate (30 µg/Lane) Lane 13: Rat liver testis lysate (30 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of MCF7 cells labeling RAB22A with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA721387

Target name :

RAB22A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human RAB22A aa 1-100 / 194.

Uniprot id :

Q9UL26>SwissProt: Q9UL26 Human;SwissProt: P35285 Mouse;Entrez Gene: 366265 Rat

Host :

Rabbit

Clone number :

JE66-08

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, Jurkat cell lysate, U-2 OS cell lysate, A549 cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse kidney tissue lysate, mouse testis tissue lysate, Rat liver testis lysate, MCF7.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Endosome membrane, Cell membrane, Early endosome, Late endosome, Cell projection, ruffle, Cytoplasmic vesicle, Cytoplasmic vesicle, phagosome, phagosome membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage