product summary
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company name :
HUABIO
product type :
antibody
product name :
HN1
catalog :
HA721384
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE55-74
reactivity :
human, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Western blot analysis of HN1 on different lysates with Rabbit anti-HN1 antibody (HA721384) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A549 cell lysate
Lane 4: K-562 cell lysate
Lane 5: HepG2 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 16 kDa
Observed band size: 20 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721384) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HN1 antibody (HA721384) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721384) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-HN1 antibody (HA721384) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721384) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA721384
Target name :
HN1
Species reactivity :
Human,Rat
Applications :
WB,ICC,IHC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human HN1 aa 105-154 / 154.
Uniprot id :
Q9UK76>SwissProt: Q9UK76 Human;SwissProt: Q6AXU6 Rat
Host :
Rabbit
Clone number :
JE55-74
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Hela cell lysate, HEK-293 cell lysate, A549 cell lysate, K-562 cell lysate, HepG2 cell lysate, human tonsil tissue, rat cerebellum tissue, A549.
Molecular wt :
Predicted band size: 16 kDa
Subcellular location :
Nucleus, Cytoplasm.
Concentration :
1mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC: 1:200
;ICC: 1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721384_4.jpg
Pic legend4 :
Immunocytochemistry analysis of A549 cells labeling HN1 with Rabbit anti-HN1 antibody (HA721384) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HN1 antibody (HA721384) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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