product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
4-1BBL
catalog :
HA721357
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE33-91
reactivity :
human, mouse
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of 4-1BBL on different lysates with Rabbit anti-4-1BBL antibody (HA721357) at 1/1,000 dilution.
Lane 1: THP-1 whole cell lysate
Lane 2: THP-1 treated with 100nM TPA overnight whole cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 29 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721357) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721357
Target name :
4-1BBL
Species reactivity :
Human,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human 4-1BBL aa 205-254 / 254.
Uniprot id :
P41273>SwissProt: P41273 Human;SwissProt: P41274 Mouse
Host :
Rabbit
Clone number :
JE33-91
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
THP-1 whole cell lysate, THP-1 treated with 100nM TPA overnight whole cell lysate, HeLa cell lysate, HepG2 cell lysate, RAW264.7 cell lysate.
Molecular wt :
Predicted band size: 27 kDa
Subcellular location :
Membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments