FADD | HUABIO HA721353 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FADD

catalog :

HA721353

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE45-25

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (HA721353) at 1/1,000 dilution. Lane 1: A431 cell lysate (10 µg/Lane) Lane 2: K-562 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: MCF7 cell lysate (10 µg/Lane) Lane 5: HeLa cell lysate (10 µg/Lane) Lane 6: SK-Br-3 cell lysate (10 µg/Lane) Lane 7: SW480 cell lysate (10 µg/Lane) Lane 8: THP-1 cell lysate (10 µg/Lane) Lane 9: A549 cell lysate (10 µg/Lane) Lane 10: HepG2 cell lysate (10 µg/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721353) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of SW480 cells labeling FADD with Rabbit anti-FADD antibody (HA721353) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FADD antibody (HA721353) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-FADD antibody (HA721353) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721353) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721353

Target name :

FADD

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human FADD aa 1-170 / 208.

Uniprot id :

Q13158>SwissProt: Q13158 Human

Host :

Rabbit

Clone number :

JE45-25

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, K-562 cell lysate, Jurkat cell lysate, MCF7 cell lysate, HeLa cell lysate, SK-Br-3 cell lysate, SW480 cell lysate, THP-1 cell lysate, A549 cell lysate, HepG2 cell lysate, SW480, human stomach tissue.

Molecular wt :

Predicted band size: 23 kDa

Subcellular location :

Cytoplasm, cytosol, neuron projection, nucleus, plasma membrane, ripoptosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200 ;IF-Cell: 1:50

more info or order :

HUABIO product webpage