Arp2 | HUABIO HA721348 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Arp2

catalog :

HA721348

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE40-62

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Arp2 on different lysates with Rabbit anti-Arp2 antibody (HA721348) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721348) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Arp2 antibody (HA721348) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721348) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunocytochemistry analysis of SH-SY5Y cells labeling Arp2 with Rabbit anti-Arp2 antibody (HA721348) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Arp2 antibody (HA721348) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA721348

Target name :

Arp2

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Arp2 aa 345-394 / 394.

Uniprot id :

P61160>SwissProt: P61160 Human;SwissProt: P61161 Mouse;SwissProt: Q5M7U6 Rat

Host :

Rabbit

Clone number :

JE40-62

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, human heart tissue, SH-SY5Y, rat spleen tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Cytoplasm, cytoskeleton, Cell projection, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:250

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721348_4.jpg

Pic legend4 :

Western blot analysis of Arp2 on different lysates with Rabbit anti-Arp2 antibody (HA721348) at 1/1,000 dilution. Lane 1: Rat spleen tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 2 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721348) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721348_5.jpg

Pic legend5 :

Western blot analysis of Arp2 on different lysates with Rabbit anti-Arp2 antibody (HA721348) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C2C12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721348) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage