RTN3 | HUABIO HA721342 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RTN3

catalog :

HA721342

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-40

reactivity :

human, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA721342) at 1/1,000 dilution. Lane 1: HeLa cell lysate (32 µg/Lane) Lane 2: HEK-293 cell lysate (33 µg/Lane) Lane 3: U87-MG cell lysate (30 µg/Lane) Lane 4: A549 cell lysate (26 µg/Lane) Lane 5: SH-SY5Y cell lysate (18 µg/Lane) Lane 6: PC-12 cell lysate (23 µg/Lane) Lane 7: L6 cell lysate (14 µg/Lane) Lane 8: Human cerebellum tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721342) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA721342) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RTN3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721342) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of PC-12 cells labeling RTN3 with Rabbit anti-RTN3 antibody (HA721342) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RTN3 antibody (HA721342) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA721342

Target name :

RTN3

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human RTN3 aa 1-500 / 1,032.

Uniprot id :

O95197>SwissProt: O95197 Human;SwissProt: Q6RJR6 Rat

Host :

Rabbit

Clone number :

PSH0-40

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, U87-MG cell lysate, A549 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, L6 cell lysate, human cerebellum tissue lysate, PC-12, HeLa.

Molecular wt :

Predicted band size: 113 kDa

Subcellular location :

Endoplasmic reticulum membrane, Golgi apparatus membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50 ;FC: 1:500-1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721342_4.jpg

Pic legend4 :

Flow cytometric analysis of HeLa cells labeling RTN3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721342, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage