Cofilin | HUABIO HA721340 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cofilin

catalog :

HA721340

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-38

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (HA721340) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 µg/Lane) Lane 2: HEK-293 cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: MDA-MB-468 cell lysate (24 µg/Lane) Lane 5: K-562 cell lysate (16 µg/Lane) Lane 6: SH-SY5Y cell lysate (22 µg/Lane) Lane 7: HUVEC cell lysate (30 µg/Lane) Lane 8: Jurkat cell lysate (30 µg/Lane) Lane 9: COS-1 cell lysate (30 µg/Lane) Lane 10: VERO cell lysate (30 µg/Lane) Lane 11: NIH/3T3 cell lysate (22 µg/Lane) Lane 12: PC-12 cell lysate (30 µg/Lane) Lane 13: Neuro-2a cell lysate (19 µg/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721340) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721340, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Flow cytometric analysis of NIH/3T3 cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721340, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA721340

Target name :

Cofilin

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Cofilin aa 117-166 / 166.

Uniprot id :

P23528>SwissProt: P23528 Human;SwissProt: P18760 Mouse;SwissProt: P45592 Rat

Host :

Rabbit

Clone number :

PSH0-38

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, MCF7 cell lysate, MDA-MB-468 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, Jurkat cell lysate, COS-1 cell lysate, VERO cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Neuro-2a cell lysate, HeLa, NIH/3T3.

Molecular wt :

Predicted band size: 19 kDa

Subcellular location :

Nucleus matrix, Cytoplasm, Cytoskeleton, Cell projection.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000;FC: 1:200-1:1,000

more info or order :

HUABIO product webpage