GABA B Receptor 1 | HUABIO HA721338 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GABA B Receptor 1

catalog :

HA721338

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE60-09

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: SHG-44 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721338) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721338) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721338, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

product information

SKU :

HA721338

Target name :

GABA B Receptor 1

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human GABA B Receptor 1 aa 35-134 / 961.

Uniprot id :

Q9UBS5>SwissProt: Q9UBS5 Human;SwissProt: Q9WV18 Mouse

Host :

Rabbit

Clone number :

JE60-09

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, SHG-44 cell lysate, mouse brain tissue.

Molecular wt :

Predicted band size: 108 kDa

Subcellular location :

Cell membrane, Postsynaptic cell membrane, Cell projection, dendrite; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:100

more info or order :

HUABIO product webpage