product summary
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company name :
HUABIO
product type :
antibody
product name :
KDM4A
catalog :
HA721336
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE36-54
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of KDM4A on different lysates with Rabbit anti-KDM4A antibody (HA721336) at 1/1,000 dilution.
Lane 1: MG-63 cell lysate (20 µg/Lane)
Lane 2: Mouse cerebellum tissue lysate (30 µg/Lane)
Lane 3: Mouse brain tissue lysate (30 µg/Lane)
Lane 4: Rat brain tissue lysate (30 µg/Lane)
Predicted band size: 121 kDa
Observed band size: 121 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721336) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of KDM4A on different lysates with Rabbit anti-KDM4A antibody (HA721336) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-KDM4A KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 120 kDa
Observed band size: 150 kDa
Exposure time: 60 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721336) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721336
Target name :
KDM4A
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human KDM4A aa 551-650 / 1,064.
Uniprot id :
O75164>SwissProt: O75164 Human;SwissProt: Q8BW72 Mouse;Entrez Gene: 313539 Rat
Host :
Rabbit
Clone number :
JE36-54
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
MG-63 cell lysate, mouse cerebellum tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
Molecular wt :
Predicted band size: 121 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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