product summary
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company name :
HUABIO
product type :
antibody
product name :
Merlin
catalog :
HA721320
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE31-02
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721320) at 1/500 dilution.
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Mouse brain tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721320) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721320) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Merlin cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721320) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721320
Target name :
Merlin
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Merlin aa 546-595 / 595.
Uniprot id :
P35240>SwissProt: P35240 Human;SwissProt: P46662 Mouse;SwissProt: Q63648 Rat
Host :
Rabbit
Clone number :
JE31-02
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Hela cell lysate, mouse brain tissue lysate, rat brain tissue lysate.
Molecular wt :
Predicted band size: 70 kDa
Subcellular location :
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:1,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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