EBP1 | HUABIO HA721315 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

EBP1

catalog :

HA721315

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE35-48

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of EBP1 on different lysates with Rabbit anti-EBP1 antibody (HA721315) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721315) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EBP1 antibody (HA721315) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721315) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-EBP1 antibody (HA721315) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721315) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721315

Target name :

EBP1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human EBP1 aa 325-374 / 394.

Uniprot id :

Q9UQ80>SwissProt: Q9UQ80 Human;SwissProt: P50580 mouse;SwissProt: Q6AYD3

Host :

Rabbit

Clone number :

JE35-48

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K562 cell lysate, HEK-293 cell lysate, PC-12 cell lysate, mouse cerebellum tissue, rat brain tissue, 293, NIH/3T3, PC-12.

Molecular wt :

Predicted band size: 44 kDa

Subcellular location :

Cytoplasm, Nucleus

Concentration :

1 mg/ml

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:50

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721315_4.jpg

Pic legend4 :

Immunocytochemistry analysis of 293 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721315_5.jpg

Pic legend5 :

Immunocytochemistry analysis of NIH/3T3 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721315_6.jpg

Pic legend6 :

Immunocytochemistry analysis of PC-12 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

more info or order :

HUABIO product webpage