MAVS | HUABIO HA721310 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MAVS

catalog :

HA721310

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-30

reactivity :

human, mouse

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MAVS on different lysates with Rabbit anti-MAVS antibody (HA721310) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: C2C12 cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: THP-1 cell lysate Lane 8: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 75 kDa The molecular weight observed is consistent with that described in the literature (PMID: 16125763 and 30460894) Exposure time: 10S; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721310) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MAVS antibody (HA721310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX

image 3 :

Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-MAVS antibody (HA721310) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721310) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX

product information

SKU :

HA721310

Target name :

MAVS

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human MAVS aa 2-50.

Uniprot id :

Q7Z434>SwissProt: Q7Z434 Human;SwissProt: Q8VCF0 Mouse

Host :

Rabbit

Clone number :

PSH0-30

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, C2C12 cell lysate, human colon tissue, human endometrium tissue, A431 cell.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Concentration :

1 mg/mL

Recommended dilutions :

WB:1:1,000 ;IHC-P: 1:200-1:1,000 ;IF-Cell: 1:100 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721310_4.png

Pic legend4 :

Immunocytochemistry analysis of A431 cells labeling MAVS with Rabbit anti-MAVS antibody (HA721310) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MAVS antibody (HA721310) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721310_5.jpg

Pic legend5 :

MAVS was immunoprecipitated from 0.2 mg A431 cell lysate with HA721310 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721310 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA721310 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA721310 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 47 seconds; ECL: K1801

more info or order :

HUABIO product webpage