product summary
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company name :
HUABIO
product type :
antibody
product name :
SEC62
catalog :
HA721305
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH0-25
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
HUABIO HA721305 image 1
Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (HA721305) at 1/5,000 dilution. Lane 1: Hela cell lysate Lane 2: Hela cell lysate Lane 3: K562 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: HepG2 cell lysate Lane 6: 293T cell lysate Lane 7: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 56 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721305) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA721305 image 2
Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (HA721305) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 56 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721305) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721305
Target name :
SEC62
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human SEC62 aa 1-50 / 399.
Uniprot id :
Q99442>SwissProt: Q99442 Human;SwissProt: Q8BU14 Mouse;Entrez Gene: 294912 Rat
Host :
Rabbit
Clone number :
PSH0-25
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Hela cell lysate, K562 cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, 293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue lysate, mouse brain tissue lysate.
Molecular wt :
Predicted band size: 46 kDa
Subcellular location :
Endoplasmic reticulum membrane.
Concentration :
1mg/mL.
Recommended dilutions :
WB: 1:5,000
more info or order :
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!