product summary
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company name :
HUABIO
product type :
antibody
product name :
ICOSLG / B7-H2 / CD275
catalog :
HA721299
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE52-85
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of ICOSLG / B7-H2 / CD275 on different lysates with Rabbit anti-ICOSLG / B7-H2 / CD275 antibody (HA721299) at 1/1,000 dilution.
Lane 1: JAR cell lysate
Lane 2: Jurkat cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 60 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721299) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of ICOSLG / B7-H2 / CD275 on different lysates with Rabbit anti-ICOSLG / B7-H2 / CD275 antibody (HA721299) at 1/500 dilution.
Lane 1: HUVEC cell lysate
Lane 2: HUVEC treated with 20ng/mL TNF-alpha for 24 hours cell lysate
Lane 3: Daudi cell lysate
Lane 4: Raji cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 65 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721299) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721299
Target name :
ICOSLG / B7-H2 / CD275
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human ICOSLG aa 250-302.
Uniprot id :
O75144>SwissProt: O75144 Human
Host :
Rabbit
Clone number :
JE52-85
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
JAR cell lysate, HUVEC cell lysate, HUVEC treated with 20ng/mL TNF-alpha for 24 hours cell lysate, Daudi cell lysate, Raji cell lysate.
Molecular wt :
Predicted band size: 33 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Cell treatment (CT),Relative expression (RE)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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