Human IL-6 | HUABIO HA721293 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human IL-6

catalog :

HA721293

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

HRP

clone name :

PS00-15

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Dilute the Capture Antibody (HA721167) to the working concentration 5μg/ml in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at 4℃. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Add human IL6 protein (HA210797) with 1:1 serially diluted starting from a concentration of 5,000pg/ml and incubate 45 minutes at room temperature. Add 100 μL of the HRP-conjugated Detection Antibody (PS00-15) at a dilution of 0.2μg/ml to each well. Cover the plate and incubate for 30 minutes at room temperature. Add 50 μL of Stop Solution to each well. Determine the optical density of each well immediately, using a microplate reader set to 450 nm.

image 2 :

Advantages of Using rAbs： Increased Reproducibility Because recombinant antibody production involves sequencing the antibody light and heavy chains, recombinant antibody production allows researchers more control over the antigen. Ease of Scalability and Continuous Supply In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. Recombinant antibodies can be produced in weeks as opposed to months. Animal-Free Tech Once the antibody-producing genes are isolated, high-throughput in vitro manufacture can be implemented. This eliminates the numerous ethical and animal welfare concerns commonly associated with traditional monoclonal antibody production.

product information

SKU :

HA721293

Target name :

Human IL-6

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

HRP-conjugated

Immunogen :

Recombinant full length of human IL6 protein (HA210797).

Uniprot id :

P05231>Entrez Gene: 3569 Human;Omim: 147620 Human;SwissProt: P05231 Human;Unigene: 654458 Human

Host :

Rabbit

Clone number :

PS00-15

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant human IL6 protein (HA210797).

Molecular wt :

Predicted band size: 24 kDa

Subcellular location :

Extracellular region or secreted.

Concentration :

1mg/ml

Recommended dilutions :

ELISA (Det): 1:10,000. Use at an assay dependent concentration.

more info or order :

HUABIO product webpage