product summary
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company name :
HUABIO
product type :
antibody
product name :
Pyruvate Dehydrogenase E2
catalog :
HA721267
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE34-38
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/1,000 dilution.
Lane 1: HL-60 cell lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (40 µg/Lane)
Predicted band size: 69 kDa
Observed band size: 60 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/2,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Pyruvate Dehydrogenase E2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721267
Target name :
Pyruvate Dehydrogenase E2
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human Pyruvate Dehydrogenase E2 aa 574-624/647.
Uniprot id :
P10515>SwissProt: P10515 Human;SwissProt: Q8BMF4 Mouse;SwissProt: P08461 Rat
Host :
Rabbit
Clone number :
JE34-38
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HL-60 cell lysate, Mouse heart tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HAP1 cell lysate, human testis tissue, mouse cerebellum tissue, rat brain tissue, rat hippocampus tissue, HepG2.
Molecular wt :
Predicted band size: 69 kDa
Subcellular location :
Mitochondrion matrix.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;IHC-P: 1:200
;IF-Cell: 1:100
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721267_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA721267_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA721267_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA721267_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA721267_8.jpg
Pic legend8 :
Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Dehydrogenase E2 with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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