MMAE | HUABIO HA721257-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MMAE

catalog :

HA721257-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-04

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Indirect ELISA analysis of MMAE was performed by coating wells of a 96-well plate with 50 µl per well of MMAE-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of MMAE monoclonal antibody (HA721257) serial diluted starting from a concentration of 20ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Indirect ELISA analysis of MMAE was performed by coating wells of a 96-well plate with 50 µl per well of MMAE-ADC diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of MMAE monoclonal antibody (HA721257) serial diluted starting from a concentration of 20ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Competitive ELISA analysis of MMAE was performed by coating wells of a 96-well plate with 50 µl per well of MMAE-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of MMAE monoclonal antibody (HA721257) at concentration of 1 µg/mL with serial diluted MMAE starting from a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

product information

SKU :

HA721257-50UL

Target name :

MMAE

Species reactivity :

Species independent

Applications :

ELISA

Conjugate :

Non-conjugated

Immunogen :

MMAE-OVA

Uniprot id :

Host :

Rabbit

Clone number :

PSH0-04

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Concentration :

1mg/ml

Recommended dilutions :

ELISA: 1:10,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721257_4.jpg

Pic legend4 :

Competitive ELISA analysis of MMAE was performed by coating wells of a 96-well plate with 50 µl per well of MMAE-ADC diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of MMAE monoclonal antibody (HA721257) at concentration of 1 µg/mL with serial diluted MMAE starting from a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721257_5.jpg

Pic legend5 :

Indirect ELISA analysis of MMAE was performed by coating wells of a 96-well plate with 50 µl per well of MMAE-BSA / MMAF-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of MMAE monoclonal antibody (HA721257) serial diluted starting from a concentration of 20ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

more info or order :

HUABIO product webpage