Ki67 | HUABIO HA721254 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ki67

catalog :

HA721254

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-02

reactivity :

human

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue with Rabbit anti-Ki67 antibody (HA721254) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721254) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Ki67 antibody (HA721254) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721254) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Ki67 antibody (HA721254) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721254) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721254

Target name :

Ki67

Species reactivity :

Human

Applications :

IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Ki67 aa 1000-1200.

Uniprot id :

P46013>SwissProt: P46013 Human

Host :

Rabbit

Clone number :

PSH0-02

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human cervix carcinoma tissue, human colon carcinoma tissue, human lymph nodes tissue, A431, Hela.

Molecular wt :

Predicted band size: 359 kDa

Subcellular location :

Chromosome, Nucleus, nucleolus.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:2,000 ;FC: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721254_4.jpg

Pic legend4 :

Flow cytometric analysis of A431 cells labeling Ki67. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721254, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721254_5.jpg

Pic legend5 :

Flow cytometric analysis of Hela cells labeling Ki67. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721254, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage