Smad4 | HUABIO HA721234 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Smad4

catalog :

HA721234

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PD01-37

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Smad4 on different lysates with Rabbit anti-Smad4 antibody (HA721234) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Ramos cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721234) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of PANC-1 cells labeling Smad4 with Rabbit anti-Smad4 antibody (HA721234) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Smad4 antibody (HA721234) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Smad4 antibody (HA721234) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721234) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721234

Target name :

Smad4

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Smad4 aa 500 to the C-terminus (C terminal).

Uniprot id :

Q13485>SwissProt: Q13485 Human;SwissProt: P97471 Mouse;SwissProt: O70437 Rat

Host :

Rabbit

Clone number :

PD01-37

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, PANC-1, human lung carcinoma tissue, human testis tissue, human stomach carcinoma tissue.

Molecular wt :

Predicted band size: 60 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:50

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721234_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Smad4 antibody (HA721234) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721234) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721234_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Smad4 antibody (HA721234) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721234) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage