product summary
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company name :
HUABIO
product type :
antibody
product name :
hSET1/SET1
catalog :
HA721226
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PH00-02
reactivity :
human, mouse
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of hSET1/SET1 on 293T cell lysates with Rabbit anti-hSET1/SET1 antibody (HA721226) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 186 kDa
Observed band size: 300 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721226) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Flow cytometric analysis of 293T cells labeling hSET1/SET1.
Cells were fixed and permeabilized.Then stained with the primary antibody (HA721226, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
image 3 :
Flow cytometric analysis of HepG2 cells labeling hSET1/SET1.
Cells were fixed and permeabilized.Then stained with the primary antibody (HA721226, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA721226
Target name :
hSET1/SET1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within C terminal human SET1A.
Uniprot id :
O15047>SwissProt: O15047 Human;SwissProt: E9PYH6 Mouse;SwissProt: A0A8I6AS32 Rat
Host :
Rabbit
Clone number :
PH00-02
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T cell lysates, 293T, HepG2.
Molecular wt :
Predicted band size: 186 kDa
Subcellular location :
Chromosome, Nucleus
Concentration :
1 mg/ml
Recommended dilutions :
WB: 1:1,000
;FC: 1:500-1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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