product summary
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company name :
HUABIO
product type :
antibody
product name :
VPAC1
catalog :
HA721209
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PD00-96
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: Caco-2 cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: LNCaP cell lysate (20 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 55 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721209) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: C2C12 cell lysate (20 µg/Lane)
Lane 3: COS-1 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: L6 cell lysate (20 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Mouse lung tissue lysate (40 µg/Lane)
Lane 8: Mouse small intestine tissue lysate (40 µg/Lane)
Lane 9: Mouse colon tissue lysate (40 µg/Lane)
Lane 10: Rat lung tissue lysate (40 µg/Lane)
Lane 11: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721209) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721209
Target name :
VPAC1
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human VIPR1 aa 400-457.
Uniprot id :
P32241>SwissProt: P32241 Human;SwissProt: P97751 Mouse;SwissProt: P30083 Rat
Host :
Rabbit
Clone number :
PD00-96
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, HT-29 cell lysate, Caco-2 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, PC-12 cell lysate, LNCaP cell lysate, human tonsil tissue, NIH/3T3 cell lysate, C2C12 cell lysate, COS-1 cell lysate, C6 cell lysate, L6 cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, mouse small intestine tissue lysate, mouse colon tissue lysate, rat lung tissue lysate, rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue, rat spleen tissue.
Molecular wt :
Predicted band size: 52 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:500-1:4,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721209_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA721209_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA721209_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA721209_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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