MAGEA1 | HUABIO HA721197 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MAGEA1

catalog :

HA721197

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PD00-50

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MAGEA1 on A431 cell lysates with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721197) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721197) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MAGEA1 antibody (HA721197) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721197) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721197

Target name :

MAGEA1

Species reactivity :

Human

Applications :

WB,IHC-P,IP,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide

Uniprot id :

P43355>SwissProt: P43355 Human

Host :

Rabbit

Clone number :

PD00-50

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysates, human malignant melanoma tissue, human testis tissue.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,500 ;IP: 1-2μg/sample ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721197_4.jpg

Pic legend4 :

MAGEA1 was immunoprecipitated from 0.2 mg A431 cell lysate with HA721197 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721197 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA721197 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA721197 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721197_5.jpg

Pic legend5 :

Application: IF-Tissue Species: Human Site: testis Sample: Paraffin-embedded section Antibody concentration: 1/500

more info or order :

HUABIO product webpage