product summary
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company name :
HUABIO
product type :
antibody
product name :
NEDD4
catalog :
HA721191
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE35-58
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (HA721191) at 1/2,000 dilution.
Lane 1: U87MG cell lysate
Lane 2: A549 cell lysate
Lane 3: NIH/3T3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 149 kDa
Observed band size: 110 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721191) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (HA721191) at 1/500 dilution.
Lane 1: Rat skin tissue lysate
Lane 2: Human skin tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 149 kDa
Observed band size: 110 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721191) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721191
Target name :
NEDD4
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant fragment corresponding to Human NEDD4 aa 160-460.
Uniprot id :
P46934>SwissProt: P46934 Human;SwissProt: P46935 Mouse;SwissProt: Q62940 Rat
Host :
Rabbit
Clone number :
JE35-58
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
U87MG cell lysate, A549 cell lysate, NIH/3T3 cell lysate, rat skin tissue lysate, human skin tissue lysate.
Molecular wt :
Predicted band size: 149 kDa
Subcellular location :
Cytoplasm, Cell membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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