Tbx3 | HUABIO HA721184 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Tbx3

catalog :

HA721184

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE33-38

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Tbx3 on JAR cell lysates with Rabbit anti-Tbx3 antibody (HA721184) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721184) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of HepG2 cells labeling Tbx3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721184, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA721184

Target name :

Tbx3

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Tbx3 aa 694-743 / 743.

Uniprot id :

O15119>SwissProt: O15119 Human

Host :

Rabbit

Clone number :

JE33-38

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

JAR cell lysates, HepG2.

Molecular wt :

Predicted band size: 79 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;FC: 1:500-1:1,000

more info or order :

HUABIO product webpage