HER2 / ErbB2 | HUABIO HA721178 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HER2 / ErbB2

catalog :

HA721178

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PD00-53

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721178) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

image 3 :

Western blot analysis of HER2 / ErbB2 on SK-Br-3 cell lysates with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 138 kDa Observed band size: 250 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721178) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721178

Target name :

HER2 / ErbB2

Species reactivity :

Human

Applications :

IHC-P,WB,IF-Cell,FC,mIHC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ErbB2/ HER2 aa 500-650.

Uniprot id :

P04626>SwissProt: P04626 Human

Host :

Rabbit

Clone number :

PD00-53

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human breast carcinoma tissue, SK-Br-3 cell lysates, MCF-7, SK-Br-3, human ovarian cancer tissue.

Molecular wt :

Predicted band size: 138 kDa

Subcellular location :

Cell membrane, Nucleus, Cytoplasm.

Concentration :

1 mg/ml

Recommended dilutions :

IHC-P: 1:2,000 ;WB: 1:1,000 ;IF-Cell: 1:50 ;FC: 1:1,000 ;mIHC: 1:2,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721178_4.jpg

Pic legend4 :

Immunocytochemistry analysis of MCF-7 cells labeling HER2 / ErbB2 with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721178_5.jpg

Pic legend5 :

Immunocytochemistry analysis of SK-Br-3 cells labeling HER2 / ErbB2 with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721178_6.jpg

Pic legend6 :

Flow cytometric analysis of SK-Br-3 cells labeling HER2 / ErbB2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721178, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA721178_7.jpg

Pic legend7 :

mIHC analysis of human ovarian cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-HER2 / ErbB2 antibody (HA721178) at 1/2,000 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

more info or order :

HUABIO product webpage