product summary
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company name :
HUABIO
product type :
antibody
product name :
CD13
catalog :
HA721160
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PD00-39
reactivity :
human, mouse
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD13 antibody (HA721160) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 2 :
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD13 antibody (HA721160) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD13 antibody (HA721160) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA721160
Target name :
CD13
Species reactivity :
Human,Mouse
Applications :
IHC-P,IF-Tissue,WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within N Terminal human CD13.
Uniprot id :
P15144>SwissProt: P15144 Human;SwissProt: P97449 Mouse
Host :
Rabbit
Clone number :
PD00-39
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Human tonsil tissue, human spleen tissue, human kidney tissue, mouse kidney tissue, PANC-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate.
Molecular wt :
Predicted band size: 110 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-P: 1:1,000-1:20,000
;IF-Tissue: 1:1,000
;WB: 1:2,000
Advanced Validation :
Relative expression (RE),Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721160_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD13 antibody (HA721160) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA721160_5.jpg
Pic legend5 :
Application: IF-Tissue
Species: Mouse
Site: kidney
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA721160_6.jpg
Pic legend6 :
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA721160) at 1/2,000 dilution.
Lane 1: PANC-1 cell lysate (5 µg/Lane)
Lane 2: THP-1 cell lysate (30 µg/Lane)
Lane 3: U-937 cell lysate (20 µg/Lane)
Lane 4: 293T cell lysate (negative) (20 µg/Lane)
Lane 5: HL-60 cell lysate (20 µg/Lane)
Lane 6: Mouse kidney tissue lysate (20 µg/Lane)
Predicted band size: 110 kDa
Observed band size: 150 kDa
Exposure time: Lane 1: 9 seconds; Lane 2-5: 24 seconds; Lane 6: 1 minute 45 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA721160_7.jpg
Pic legend7 :
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA721160) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD13 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 150 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721160) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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