product summary
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company name :
HUABIO
product type :
antibody
product name :
TGF beta 1
catalog :
HA721143-50UL
quantity :
50μl
price :
205.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PD00-17
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA721143-50UL image 1
Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/5,000 dilution. Lane 1: K-562 cell lysate Lane 2: Raji cell lysate Lane 3: Saos-2 cell lysate Lane 4: 786-0 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: L-929 cell lysate Lane 9: C6 cell lysate Lane 10: Mouse spleen tissue lysate Lane 11: Mouse skeletal muscle tissue lysate (negative) Lane 12: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 48/13 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721143) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA721143-50UL image 2
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
HUABIO HA721143-50UL image 3
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA721143-50UL
Target name :
TGF beta 1
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide (KLH-coupled) corresponding to Human TGF-Beta 1 C-terminal.
Uniprot id :
P01137>SwissProt: P01137 Human;SwissProt: P04202 Mouse;SwissProt: P17246 Rat
Host :
Rabbit
Clone number :
PD00-17
Isotype :
IgG
Size :
50μl
List Price :
205.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
K-562 cell lysate, Raji cell lysate, Saos-2 cell lysate, 786-0 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, L-929 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, rat spleen tissue, mouse spleen tissue.
Molecular wt :
Predicted band size: 44 kDa
Subcellular location :
Extracellular matrix, Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000 ;IHC-P: 1:200 ;IF-Tissue: 1:200
Advanced Validation :
Relative expression (RE)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!