RICTOR | HUABIO HA721141 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RICTOR

catalog :

HA721141

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

1D5

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RICTOR on different lysates with Rabbit anti-RICTOR antibody (HA721141) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: SW480 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 192 kDa Observed band size: 192 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721141) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of A431 cells labeling RICTOR with Rabbit anti-RICTOR antibody (HA721141) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RICTOR antibody (HA721141) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Flow cytometric analysis of Hela cells labeling RICTOR. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721141, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA721141

Target name :

RICTOR

Species reactivity :

Human

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human RICTOR aa 1459-1708 / 1708.

Uniprot id :

Q6R327>SwissProt: Q6R327 Human

Host :

Rabbit

Clone number :

1D5

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysate, SW480 cell lysate, A431, Hela.

Molecular wt :

Predicted band size: 192 kDa

Subcellular location :

Cytosol, Golgi apparatus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:100 ;FC: 1:500-1:1,000

more info or order :

HUABIO product webpage