GAPDH | HUABIO HA721131 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GAPDH

catalog :

HA721131

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

HRP

clone name :

SA30-01

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GAPDH on HepG2 cell lysates with Rabbit anti-GAPDH antibody (HA721131) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

image 2 :

Western blot analysis of GAPDH on PC-12 cell lysates with Rabbit anti-GAPDH antibody (HA721131) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GAPDH antibody (HA721131) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721131) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721131

Target name :

GAPDH

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC

Conjugate :

HRP-conjugated

Immunogen :

Recombinant protein within mouse GAPDH aa 94-333 / 333.

Uniprot id :

P04406>SwissProt: P04406 Human;SwissProt: P16858 Mouse;SwissProt: P04797 Rat

Host :

Rabbit

Clone number :

SA30-01

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysates, PC-12 cell lysates, NIH/3T3 cell lysates, THP-1 cell lysate, MCF-7 cell lysate, RH-35 cell lysate, HepG2 cell lysate, PC-12 cell lysate, L6 cell lysate, RAW264.7 cell lysate, A549 cell lysate, PMVEC cell lysate, NIH/3T3 cell lysate, B16F1 cell lysate.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Cytoplasm, Nucleus, Membrane.

Concentration :

0.5 mg/mL.

Recommended dilutions :

WB: 1:10,000-1:20,000 ;IHC:1:200-1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721131_4.jpg

Pic legend4 :

Western blot analysis of GAPDH on NIH/3T3 cell lysates with Rabbit anti-GAPDH antibody (HA721131) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721131_5.jpg

Pic legend5 :

Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (HA721131) at 1/10,000 dilution. Lane 1: THP-1 cell lysate Lane 2: MCF-7 cell lysate Lane 3: RH-35 cell lysate Lane 4: HepG2 cell lysate Lane 5: PC-12 cell lysate Lane 6: L6 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: A549 cell lysate Lane 9: PMVEC cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: B16F1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721131_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GAPDH antibody (HA721131) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721131) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA721131_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GAPDH antibody (HA721131) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721131) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage